Description: Human Anti-Salmonella IgG Antibody Assay Kit

Description: A competitive ELISA for quantitative measurement of Human True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Human True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a kit designed to measure DNA complex formations of PARP1 and PARP2 enzymes in a high throughput screening assay using fluorescence polarization (FP). The key to the PARPtrap™ Combo Assay Kit are the fluorescent-labeled DNA probes recognized by PARP1 and PARP2. In the absence of ribosylation, PARP1/2 binds to the DNA fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after autoribosylation, PARP1/2 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP inhibitor results in trapping of PARP1/2 to the fluorescent-labeled DNA, and increases the FP signal in a dose dependent manner.The combo kit is particularly useful to directly and specifically compare, in a single assay, the effect and potency of a compound on PARP1 and PARP2. The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a homogeneous fluorescence polarization assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.

Description: Mouse Anti-LPS IgG Antibody Assay Kit

Description: Mouse Anti-SEB IgG Antibody Assay Kit

Description: Mouse Anti-SEA IgG Antibody Assay Kit

Description: Mouse Anti-dsDNA IgG Antibody Assay Kit

Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

Description: MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect MDA Competitive ELISA Kit provides a sensitive, specific method for detection of this lipid peroxidation by-product. Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.

Description: 4-hydroxynonenal (4-HNE or HNE) is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. Our OxiSelect HNE Adduct Competitive ELISA Kit measures the formation of HNE adducts to any protein residue using a competitive ELISA format.

Description: The OxiSelect Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts.  The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve.  Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.

Description: The OxiSelect Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts.  The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve.  Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.

Description: Glutathione helps protect cells from free radical damage as an antioxidant, but it can also covalently attach to proteins through its sulfhydryl moiety upon derivatization by reactive oxygen species (ROS). Glutathione protein adducts can therefore serve as a marker of oxidative stress. OxiSelect s-Glutathione Adduct Competitive ELISA Kit quantifies the glutathionylation of proteins in a standard 96-well plate.

Description: First, a CEL conjugate is coated on the ELISA plate.  The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate.  After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody.  The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve.  Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect CEL ELISA Kit will not cross react with CML protein adducts.

Description: Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins. The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage. The OxiSelect N-epsilon-(Carboxymethyl) Lysine Competitive ELISA kit specifically detects CML formation with a high level of sensitivity. This is a Competitive ELISA Kit in which the plate is coated with a CML conjugate.  Standards and unknown samples are added to the plate, followed by incubation with the primary antibody. The CML in the unknown samples and the CML attached to the plate compete for the primary antibody. Higher CML content in unknown samples results in more binding of the antibody to the sample, and thus less antibody binds to the plate. Since the antibody bound to the sample is washed away, higher CML content in samples correlates with a lower signal.