SIRT4 suppresses the PI3K/Akt/NF‑κB signaling pathway and attenuates HUVEC injury induced by oxLDL.
Atherosclerosis is a chronic and progressive disease. Its morbidity and mortality have shown an increase in recent years. This study aims to explore the role of sirtuins (SIRT) 4 in the progression of atherosclerosis. Changes in the expression SIRT4 in response to oxidized low density lipoprotein (oxLDL) is measured in human umbilical vein endothelial cells (HUVECs) using western blotting. Cell counting kit-8 and fl ow cytometry tests were used to explore the effects of SIRT4 on HUVEC proliferation and apoptosis.
SIRT4 influence on the expression of inflammatory factors in HUVECs was analyzed using ELISA. Expression and phosphorylation of proteins in the phosphoinositide 3-kinase (PI3K) / protein kinase B (Akt) / nuclear factor (NF) -κB relative paths are analyzed using western blotting. p65 nuclear translocation of NF-kB examined using immunofluorescence. This study showed that treatment decreased the expression SIRT4 oxLDL in HUVECs in a dose-and time-dependent. SIRT4 excess promoted HUVEC oxLDL-induced cell proliferation and apoptosis is inhibited.
Furthermore, excessive SIRT4 suppress the PI3K / Akt / NF-kB pathway by inhibiting the phosphorylation of PI3K and phosphorylated (p) -Akt, p-nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor of α and p-NF-kB p65 expression; blocking NF-kB p65 nuclear translocation and reduced interleukin (IL) -1β, IL-6, and tumor necrosis factor α expression in oxLDL-induced HUVECs. In conclusion, excess SIRT4 enhanced HUVEC survival, suppress the PI3K / Akt / NF-kB signaling pathway and inhibit the inflammatory cytokine expression in oxLDL-induced HUVECs.
SIRT4 suppresses the PI3K/Akt/NF‑κB signaling pathway and attenuates HUVEC injury induced by oxLDL.
Anti-inflammatory effects of aerial bulblets extract of Dioscorea japonica Thunb through inhibition of NF-kB and MAPK signaling pathway in RAW 264.7.
Yam (Dioscorea japonica Thunb) is a well-known health food in Korea and is widely distributed in temperate and tropical regions. Although a variety of health effects from yam has been demonstrated, there is little knowledge about the properties currently Youngyeoja (VCT, the potato crop aerial bulblets), their physiological effects, and their mechanisms action.
To investigate anti-inflammatory effects of VCT, we tested the levels of inflammatory mediators in lipopolysaccharide (LPS) –stimulated RAW 264.7 cells treated with the extract of VCT. Nitric oxide (NO) and prostaglandin E2 levels (PGE2) is determined by using enzyme-linked immunosorbent assay tests. The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated by using real-time polymerase chain reaction and western blotting. In addition, activation of nuclear factor-kappaB (NF-kB) and MAPK (MAPK) were detected using western blotting.Treatment real macrophages with LPS-induced production of NO and PGE2.
Description: MM-102 is an antagonist of MLL1 with IC50 value of 2.4nM [1].Mixed lineage leukemia 1 (MLL1) is a histone H3 lysine 4 (H3K4) methyltransferase.
Description: MM-102 is an antagonist of MLL1 with IC50 value of 2.4nM [1].Mixed lineage leukemia 1 (MLL1) is a histone H3 lysine 4 (H3K4) methyltransferase.
Description: MM-102 is an antagonist of MLL1 with IC50 value of 2.4nM [1].Mixed lineage leukemia 1 (MLL1) is a histone H3 lysine 4 (H3K4) methyltransferase.
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat, Chicken. This p38 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human p38
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat, Chicken. This p38 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human p38
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat, Chicken. This p38 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human p38
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180/Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180/Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180/Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y323
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y323
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y323
Description: p38 is a protein encoded by the MAPK14 gene which is approximately 41,2 kDa. p38 is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the IL-2 pathway, toll-like receptor signalling pathways, the VEGF signalling pathway and 4-1BB pathway. This protein falls under the MAP kinase family. It acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. p38 is expressed in the brain, heart, placenta, pancreas and skeletal muscle. Mutations in the MAPK14 gene may result in patellar tendinitis and lumbosacral lipoma. STJ94878 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of p38 protein.
YYJ treatment of inflammatory mediators and inhibit the induction of the expression of iNOS and COX-2. phosphorylation, more importantly, LPS-induced nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor (IκB) was suppressed by treatment with the VFA, VFA showed inhibit the activation of NF-kB. Furthermore, VFA inhibits LPS-induced phosphorylation MAPKs.YYJ shown to have potent anti-inflammatory effects in LPS-stimulated RAW 264.7 cells, which can be attributed to an inhibitory effect on NF-kB and MAPK activation, consequently blocking the production of inflammatory factors. Therefore, these results indicate that extracts of VCT can be used as an anti-inflammatory agent.
