SIRT4 suppresses the PI3K/Akt/NF‑κB signaling pathway and attenuates HUVEC injury induced by oxLDL.
Atherosclerosis is a chronic and progressive disease. Its morbidity and mortality have shown an increase in recent years. This study aims to explore the role of sirtuins (SIRT) 4 in the progression of atherosclerosis. Changes in the expression SIRT4 in response to oxidized low density lipoprotein (oxLDL) is measured in human umbilical vein endothelial cells (HUVECs) using western blotting. Cell counting kit-8 and fl ow cytometry tests were used to explore the effects of SIRT4 on HUVEC proliferation and apoptosis.
SIRT4 influence on the expression of inflammatory factors in HUVECs was analyzed using ELISA. Expression and phosphorylation of proteins in the phosphoinositide 3-kinase (PI3K) / protein kinase B (Akt) / nuclear factor (NF) -κB relative paths are analyzed using western blotting. p65 nuclear translocation of NF-kB examined using immunofluorescence. This study showed that treatment decreased the expression SIRT4 oxLDL in HUVECs in a dose-and time-dependent. SIRT4 excess promoted HUVEC oxLDL-induced cell proliferation and apoptosis is inhibited.
Furthermore, excessive SIRT4 suppress the PI3K / Akt / NF-kB pathway by inhibiting the phosphorylation of PI3K and phosphorylated (p) -Akt, p-nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor of α and p-NF-kB p65 expression; blocking NF-kB p65 nuclear translocation and reduced interleukin (IL) -1β, IL-6, and tumor necrosis factor α expression in oxLDL-induced HUVECs. In conclusion, excess SIRT4 enhanced HUVEC survival, suppress the PI3K / Akt / NF-kB signaling pathway and inhibit the inflammatory cytokine expression in oxLDL-induced HUVECs.
SIRT4 suppresses the PI3K/Akt/NF‑κB signaling pathway and attenuates HUVEC injury induced by oxLDL.
Anti-inflammatory effects of aerial bulblets extract of Dioscorea japonica Thunb through inhibition of NF-kB and MAPK signaling pathway in RAW 264.7.
Yam (Dioscorea japonica Thunb) is a well-known health food in Korea and is widely distributed in temperate and tropical regions. Although a variety of health effects from yam has been demonstrated, there is little knowledge about the properties currently Youngyeoja (VCT, the potato crop aerial bulblets), their physiological effects, and their mechanisms action.
To investigate anti-inflammatory effects of VCT, we tested the levels of inflammatory mediators in lipopolysaccharide (LPS) –stimulated RAW 264.7 cells treated with the extract of VCT. Nitric oxide (NO) and prostaglandin E2 levels (PGE2) is determined by using enzyme-linked immunosorbent assay tests. The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated by using real-time polymerase chain reaction and western blotting. In addition, activation of nuclear factor-kappaB (NF-kB) and MAPK (MAPK) were detected using western blotting.Treatment real macrophages with LPS-induced production of NO and PGE2.
Description: Available in various conjugation types.
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YYJ treatment of inflammatory mediators and inhibit the induction of the expression of iNOS and COX-2. phosphorylation, more importantly, LPS-induced nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor (IκB) was suppressed by treatment with the VFA, VFA showed inhibit the activation of NF-kB. Furthermore, VFA inhibits LPS-induced phosphorylation MAPKs.YYJ shown to have potent anti-inflammatory effects in LPS-stimulated RAW 264.7 cells, which can be attributed to an inhibitory effect on NF-kB and MAPK activation, consequently blocking the production of inflammatory factors. Therefore, these results indicate that extracts of VCT can be used as an anti-inflammatory agent.
