The synthesis of well-defined polypeptides show complex macromolecular architecture requires the use of monomers that can be grouped by orthogonally grouped, containing primary amines that will be used as initiary for polymerization of the ring opening (ROP) anhydride n-carboxy. Synthesis and characterization of novel nε-9-fluorylmethoxycarbony-ls-carboxy anhydride (Na-FMOC-Lysine NCA), as well as linear poly novel (Nε-FMOC-L-Lys) n homopipeptide and poly (L-lysine) 78- Block- [Poly (L-Lysine) 10-graft-poly (L-Histidine) 15] Copolypeptide block-graft, served.
The copolypeptide graft synthesis was carried out through the NA-BOC-L-Lysine NCA ROP when using n-hexylamine as an initiator, followed by the polymerization of NCA Nε-FMOC-L-lysine. The latest blocks are selectively explored in basic conditions, and the resulting ε-amine is used as an initiation species for ROP NIM-Trity-L-Histidine NCA. Finally, the Board of Commissioners and Trt groups were sentenced by TFA. High vacuum techniques are applied to achieve the conditions needed for well-defined polypeptide synthesis. Molecular characterization shows that polypeptides show molecular homogeneity and high composition.
Finally, dynamic light scattering, ζ-potential, and a circular crocated measurement is used to investigate the ability of polypeptides to gather itself in different conditions. This monomer paves the way for polypeptide synthesis with complex macromolecular architecture that can define aggregation behavior, and, therefore, can cause the synthesis of responsive nanocarrier “smart” stimulation for controlled drug delivery applications.
Antimicrobial multifunctional polypeptide-selenium nanoparticles combating drug resistance bacteria
Antibiotic-resistant bacteria are severe threats to human health. The global global surveillance system of the Health Health Health has revealed antibiotic resistance between half a million patients in 22 countries, with Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae became the most resistant species. Antimicrobial nanoparticles emerged as an alternative that promised antibiotics in the fight against antimicrobial resistance. In this work, selenium nanoparticles are coated with antimicrobial polypeptides, ε-poly-licin, (SE np-ε-pl) synthesized and antibacterial activity and cytotoxicity investigated. SE NP-ε-PL which shows significantly larger antibacterial activity of all eight species of bacteria tested, including gram-positive, gram-negative strains, and drug resistance, than their individual components, SE NP and ε-pl.
Nanoparticles do not show toxicity to human dermal fibroblasts at the minimum inhibitory concentration, showing therapeutic windows. Furthermore, unlike conventional kanamycin antibiotics, SE NP-ε-PL does not easily induce resistance to E. coli or S. aureus. In particular, S. aureus began developing resistance to Kanamycin from the generation of ~ 44, while needed ~ 132 generations for resistance to develop into SE np-ε-pl. Surprisingly, E. coli cannot develop resistance to nanoparticles above ~ 300 generations. These results indicate that the multifunctional approach to combining SE NP with ε-PL to form SE NP-ε-PL is a new strategy that is very efficacious with the antibacterial activity of the width spectrum, low cytotoxicity, and significant delay in the development of resistance.
Open polypeptides open surface are successfully used as targets to develop a one-step prototype immunochromatography strip for specific and sensitive direct detection of staphylococcus aureus which causes neonatal sepsis
Neonatal sepsis is a life-threatening condition and Staphylococcus aureus is one of the main causes. However, until now, there are no fast and sensitive diagnostic tools have been developed for direct detection. Bioinformatics analysis identifies 112 amino acid polypeptides exposed to the surface of the cell protein nwmn_1649 cell walls, surface protein involved in cell aggregation and biofilm formation, as specific species and moiety which is highly preserved.

Polypeptides are cloned, purified, and used to immunize mice to increase certain immunoglobulin. Purified antibodies are conjugated to gold nano particles and are used to assemble immunochromatographic strips (ICS). The prototype IC developed is detected as low as 5 μg of pure polypeptides and 102 CFU / ML S. aureus in 15 minutes. The strip shows superior ability to directly detect S. aureus in the blood specimens of neonatal sepsis without previous sample processing. In addition, it does not show cross reactions in specimens infected with two other main causes of neonatal sepsis; Koagulase-negative Staphylococci and Klebsiella pneumoniae. The selected NWMN_1649 polypeptide shown shows success as a promising biomolecule where IC prototypes have been developed. This ICS provides fast, direct, sensitive and specific options for S. aureus detection which causes neonatal sepsis. Such tools are needed especially in limited countries of resources.
Stabilization of oil emulsion in soybean water using multilayers polypeptides: cationic polylysine and anionic acid polyglutamate
Oil-in-water emulsions are used as a shipping system for non-polar functional materials in various industries, including food, cosmetics, personal care products, agrochemicals and pharmaceuticals. However, emulsions tend to be damaged in conditions found in many commercial products. In this study, the functional performance of lipid droplets in emulsions is adjusted to electrostatic deposition of layers after the sequential layers of charged polypeptides contrary to their surface. Cationic Poly-L-lysine (PLL) and anionic poly-glutamic acid (PGA) are used as a pair of polypeptides imposed on the opposite (pH 4.0).
First, the primary emulsion (10% W / W soybean oil emulsion) is formed consisting of small lipid droplets (D32 = 500 μm) coated by natural surfactants (0.05% w / w Quilleta Saponin). Second, Cationic PLL is saved to the surface of anionic saponin-plated droplets. Third, Anionic PGA was saved to the surface of the cationic PLL-Saponin-plated droplets. We then assess the ability of coatings to protect lipid droplets from aggregation when pH (2.0-9.0), ionic power (0-350 mm), or temperature (30-90 ° C) changed. Primary, secondary and tertiary emulsion properties are monitored by measuring the average particle diameter (D32), electrical characteristics (ζ-potential), and micro from lipid droplets.
TAT Antibody |
1-CSB-PA050213 |
Cusabio |
|
|
|
Description: A polyclonal antibody against TAT. Recognizes TAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000 |
TAT Antibody |
1-CSB-PA023175LA01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against TAT. Recognizes TAT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
TAT Antibody |
1-CSB-PA024203 |
Cusabio |
|
|
|
Description: A polyclonal antibody against TAT. Recognizes TAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
Tat antibody |
70R-8790 |
Fitzgerald |
50 ug |
EUR 560.4 |
Description: Affinity purified rabbit polyclonal Tat antibody |
TAT antibody |
39162-100ul |
SAB |
100ul |
EUR 302.4 |
TAT Antibody |
35946-100ul |
SAB |
100ul |
EUR 302.4 |
TAT antibody |
10-2250 |
Fitzgerald |
200 ul |
EUR 975.6 |
Description: Mouse monoclonal TAT antibody |
TAT 14 |
B5721-1 |
ApexBio |
1 mg |
EUR 388.8 |
Human immunodeficiency virus-Tat Protein (HIV-Tat) ELISA |
201-12-1915 |
SunredBio |
96 tests |
EUR 528 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human immunodeficiency virus-Tat Protein(HIV-Tat)ELISA |
GA-E1931HM-48T |
GenAsia Biotech |
48T |
EUR 346.8 |
Human immunodeficiency virus-Tat Protein(HIV-Tat)ELISA |
GA-E1931HM-96T |
GenAsia Biotech |
96T |
EUR 559.2 |
Human immunodeficiency virus-Tat Protein(HIV-Tat)ELISA |
QY-E02393 |
Qayee Biotechnology |
96T |
EUR 433.2 |
TAT Conjugated Antibody |
C35946 |
SAB |
100ul |
EUR 476.4 |
TAT cloning plasmid |
CSB-CL023175HU-10ug |
Cusabio |
10ug |
EUR 279.6 |
|
Description: A cloning plasmid for the TAT gene. |
TAT Polyclonal Antibody |
ES7344-100ul |
ELK Biotech |
100ul |
EUR 334.8 |
Description: A Rabbit Polyclonal antibody against TAT from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA |
TAT Polyclonal Antibody |
ES7344-50ul |
ELK Biotech |
50ul |
EUR 248.4 |
Description: A Rabbit Polyclonal antibody against TAT from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA |
HIV1 tat antibody |
20-000601 |
Fitzgerald |
500 ul |
EUR 483.6 |
Description: Rabbit polyclonal HIV1 tat antibody |
HIV1 tat antibody |
20-000601F |
Fitzgerald |
500 ul |
EUR 460.8 |
Description: Rabbit polyclonal HIV1 tat antibody |
HIV1 tat antibody |
20-000602 |
Fitzgerald |
100 ug |
EUR 159.6 |
Description: Rabbit polyclonal HIV1 tat antibody |
HIV1 tat antibody |
20-HS75 |
Fitzgerald |
50 ul |
EUR 307.2 |
Description: Sheep polyclonal HIV1 tat antibody |
Sox2 TAT protein |
30R-AS056 |
Fitzgerald |
25 ug |
EUR 327.6 |
Description: Purified recombinant Human Sox2 TAT protein |
HIV1 tat protein |
30-017001F |
Fitzgerald |
100 ug |
EUR 1234.8 |
Description: Purified recombinant HIV1 tat protein |
HIV1 tat protein |
30-AH85 |
Fitzgerald |
50 ug |
EUR 1152 |
Description: Purified recombinant HIV1 tat protein |
HIV1 tat antibody |
10-007001 |
Fitzgerald |
100 ug |
EUR 483.6 |
Description: Mouse monoclonal HIV1 tat antibody |
HIV1 tat antibody |
10-H30K |
Fitzgerald |
100 ug |
EUR 697.2 |
Description: Mouse monoclonal HIV1 tat antibody |
TAT Polyclonal Antibody |
ABP56345-003ml |
Abbkine |
0.03ml |
EUR 189.6 |
|
Description: A polyclonal antibody for detection of TAT from Human, Mouse, Rat. This TAT antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human TAT |
TAT Polyclonal Antibody |
ABP56345-01ml |
Abbkine |
0.1ml |
EUR 346.8 |
|
Description: A polyclonal antibody for detection of TAT from Human, Mouse, Rat. This TAT antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human TAT |
TAT Polyclonal Antibody |
ABP56345-02ml |
Abbkine |
0.2ml |
EUR 496.8 |
|
Description: A polyclonal antibody for detection of TAT from Human, Mouse, Rat. This TAT antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human TAT |
TAT Rabbit pAb |
A6764-100ul |
Abclonal |
100 ul |
EUR 369.6 |
TAT Rabbit pAb |
A6764-200ul |
Abclonal |
200 ul |
EUR 550.8 |
TAT Rabbit pAb |
A6764-20ul |
Abclonal |
20 ul |
EUR 219.6 |
TAT Rabbit pAb |
A6764-50ul |
Abclonal |
50 ul |
EUR 267.6 |
Nanog-TAT Protein |
20-abx262148 |
Abbexa |
-
EUR 6373.20
-
EUR 393.60
-
EUR 276.00
|
|
|
TAT 2-4 |
H-7648.0500 |
Bachem |
0.5mg |
EUR 163.2 |
Description: Sum Formula: C132H240N66O29; CAS# [1159916-66-1] net |
TAT 2-4 |
H-7648.1000 |
Bachem |
1.0mg |
EUR 262.8 |
Description: Sum Formula: C132H240N66O29; CAS# [1159916-66-1] net |
SOX2-TAT, human |
RC712-46 |
Bio Basic |
5ug |
EUR 125.26 |
|
TAT stable cells |
SC012 |
GenTarget |
2 x 106 cell/ml x 1ml |
EUR 1800 |
Description: Stable cell line expressing codon humanized TAT gene and RFP with Blasticidin resistance |
Anti-TAT antibody |
STJ28847 |
St John's Laboratory |
100 µl |
EUR 332.4 |
Description: This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible mental retardation. A regulator gene for tyrosine aminotransferase is X-linked. |
Anti-TAT antibody |
STJ95908 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Rabbit polyclonal to TAT. |
Canine C-Peptide ELISA Kit |
DLR-C-Peptide-c-48T |
DL Develop |
48T |
EUR 632.4 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Canine C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Canine C-Peptide ELISA Kit |
DLR-C-Peptide-c-96T |
DL Develop |
96T |
EUR 825.6 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Canine C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human C-Peptide ELISA Kit |
DLR-C-Peptide-Hu-48T |
DL Develop |
48T |
EUR 477.6 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Human C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human C-Peptide ELISA Kit |
DLR-C-Peptide-Hu-96T |
DL Develop |
96T |
EUR 613.2 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Human C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Mouse C-Peptide ELISA Kit |
DLR-C-Peptide-Mu-48T |
DL Develop |
48T |
EUR 540 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Mouse C-Peptide ELISA Kit |
DLR-C-Peptide-Mu-96T |
DL Develop |
96T |
EUR 698.4 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat C-Peptide ELISA Kit |
DLR-C-Peptide-Ra-48T |
DL Develop |
48T |
EUR 560.4 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat C-Peptide ELISA Kit |
DLR-C-Peptide-Ra-96T |
DL Develop |
96T |
EUR 726 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat C-Peptide in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Canine C-Peptide ELISA Kit |
RDR-C-Peptide-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 668.4 |
Canine C-Peptide ELISA Kit |
RDR-C-Peptide-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 928.8 |
Human C-Peptide ELISA Kit |
RDR-C-Peptide-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 484.8 |
Human C-Peptide ELISA Kit |
RDR-C-Peptide-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 667.2 |
Mouse C-Peptide ELISA Kit |
RDR-C-Peptide-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 558 |
Mouse C-Peptide ELISA Kit |
RDR-C-Peptide-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 771.6 |
Rat C-Peptide ELISA Kit |
RDR-C-Peptide-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 583.2 |
Rat C-Peptide ELISA Kit |
RDR-C-Peptide-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 806.4 |
Canine C-Peptide ELISA Kit |
RD-C-Peptide-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 639.6 |
Canine C-Peptide ELISA Kit |
RD-C-Peptide-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 888 |
Human C-Peptide ELISA Kit |
RD-C-Peptide-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 464.4 |
Human C-Peptide ELISA Kit |
RD-C-Peptide-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 638.4 |
Mouse C-Peptide ELISA Kit |
RD-C-Peptide-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 535.2 |
Mouse C-Peptide ELISA Kit |
RD-C-Peptide-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 738 |
Rat C-Peptide ELISA Kit |
RD-C-Peptide-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 558 |
Rat C-Peptide ELISA Kit |
RD-C-Peptide-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 771.6 |
Mouse TAT shRNA Plasmid |
20-abx982152 |
Abbexa |
|
|
|
Rat TAT shRNA Plasmid |
20-abx984656 |
Abbexa |
|
|
|
Human TAT shRNA Plasmid |
20-abx954724 |
Abbexa |
|
|
|
TAT Antibody, HRP conjugated |
1-CSB-PA023175LB01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against TAT. Recognizes TAT from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
TAT Antibody, FITC conjugated |
1-CSB-PA023175LC01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against TAT. Recognizes TAT from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
TAT Antibody, Biotin conjugated |
1-CSB-PA023175LD01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against TAT. Recognizes TAT from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Sheep TAT ELISA Kit |
EST0509 |
Abclonal |
96Tests |
EUR 625.2 |
Human TAT ELISA Kit |
EHT0509 |
Abclonal |
96Tests |
EUR 625.2 |
Porcine TAT ELISA Kit |
EPT0509 |
Abclonal |
96Tests |
EUR 625.2 |
Monkey TAT ELISA Kit |
EMKT0509 |
Abclonal |
96Tests |
EUR 625.2 |
Mouse TAT ELISA Kit |
EMT0509 |
Abclonal |
96Tests |
EUR 625.2 |
Rat TAT ELISA Kit |
ERT0509 |
Abclonal |
96Tests |
EUR 625.2 |
Rabbit TAT ELISA Kit |
ERTT0509 |
Abclonal |
96Tests |
EUR 625.2 |
HIV1 tat protein (FITC) |
65-017003F |
Fitzgerald |
50 ug |
EUR 159.6 |
Description: Purified recombinant HIV1 tat protein (FITC) |
Sox2-TAT Recombinant Protein |
40-120-0005mg |
ProSci |
0.005 mg |
EUR 311.1 |
Description: Sox2, also known as sex determining region Y (SRY)-box 2, belongs to a diverse family of structurally-related transcription factors whose primary structure contains a 79-residue DNA-binding domain, called high mobility group (HMG) box. It plays an essential role in maintaining the pluripotency of embryonic stem cells (ESC) and determination of cell fate. Microarray analysis showed that Sox2 regulates the expression of multiple genes involved in embryonic development including FGF-4, YES1 and ZFP206. Sox2 acts as a transcriptional activator after forming a ternary complex with Oct3/4 and a conserved non-coding DNA sequence (CNS1) located approximately 2 kb upstream of the RAX promoter. The introduction of Sox2, Oct4, Myc, and Klf4, into human dermal fibroblasts isolated from a skin biopsy of a healthy research fellow was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology, gene expression, and in the capacity to form teratomas in immune-deficient mice. Sox2 and other transcription factors have been introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary as well as transformed cells. Recombinant human Sox2-TAT expressed in E. coli is a 36 kDa protein containing 330 amino-acid residues, including the 317 residues of full-length Sox2 and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR). |
Sox2-TAT Recombinant Protein |
40-120-0025mg |
ProSci |
0.025 mg |
EUR 437.1 |
Description: Sox2, also known as sex determining region Y (SRY)-box 2, belongs to a diverse family of structurally-related transcription factors whose primary structure contains a 79-residue DNA-binding domain, called high mobility group (HMG) box. It plays an essential role in maintaining the pluripotency of embryonic stem cells (ESC) and determination of cell fate. Microarray analysis showed that Sox2 regulates the expression of multiple genes involved in embryonic development including FGF-4, YES1 and ZFP206. Sox2 acts as a transcriptional activator after forming a ternary complex with Oct3/4 and a conserved non-coding DNA sequence (CNS1) located approximately 2 kb upstream of the RAX promoter. The introduction of Sox2, Oct4, Myc, and Klf4, into human dermal fibroblasts isolated from a skin biopsy of a healthy research fellow was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology, gene expression, and in the capacity to form teratomas in immune-deficient mice. Sox2 and other transcription factors have been introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary as well as transformed cells. Recombinant human Sox2-TAT expressed in E. coli is a 36 kDa protein containing 330 amino-acid residues, including the 317 residues of full-length Sox2 and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR). |
KLF4-TAT Recombinant Protein |
40-524-0005mg |
ProSci |
0.005 mg |
EUR 311.1 |
Description: KLF4 is a member of the Kruppel-like factor (KLF) family of zinc finger transcription factors. Members of this family have in common 3 contiguous C2H2-type zinc fingers at the carboxyl terminus that comprise the DNA-binding domain. KLF4 is highly expressed in skin and gut epithelial tissues, but is also found in various other cells and tissues, including vascular endothelial cells, lymphocytes, lung, and testis. It is an important regulator of the cell cycle, transcription, and cell differentiation. Together with Sox2, Oct4, and cMyc, KLF4 can induce the reprogramming of primary human fibroblasts to a pluripotent state. KLF4 and other transcription factors can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant Human KLF4-TAT is a 483 amino acid protein, including a 13-residue C-terminal TAT peptide, with a calculated molecular weight of 51.7kDa. Recombinant Human KLF4-TAT is a mixture of the expected sequence beginning at Met1 and a truncated isoform beginning at Tyr54. Due to post-translational modifications, SDS-PAGE gel shows bands at approximately 72 and 66kDa, under reduced conditions. |
KLF4-TAT Recombinant Protein |
40-524-0025mg |
ProSci |
0.025 mg |
EUR 437.1 |
Description: KLF4 is a member of the Kruppel-like factor (KLF) family of zinc finger transcription factors. Members of this family have in common 3 contiguous C2H2-type zinc fingers at the carboxyl terminus that comprise the DNA-binding domain. KLF4 is highly expressed in skin and gut epithelial tissues, but is also found in various other cells and tissues, including vascular endothelial cells, lymphocytes, lung, and testis. It is an important regulator of the cell cycle, transcription, and cell differentiation. Together with Sox2, Oct4, and cMyc, KLF4 can induce the reprogramming of primary human fibroblasts to a pluripotent state. KLF4 and other transcription factors can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant Human KLF4-TAT is a 483 amino acid protein, including a 13-residue C-terminal TAT peptide, with a calculated molecular weight of 51.7kDa. Recombinant Human KLF4-TAT is a mixture of the expected sequence beginning at Met1 and a truncated isoform beginning at Tyr54. Due to post-translational modifications, SDS-PAGE gel shows bands at approximately 72 and 66kDa, under reduced conditions. |
Nanog-TAT Recombinant Protein |
40-526-0005mg |
ProSci |
0.005 mg |
EUR 311.1 |
Description: Nanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of Nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of Nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, which helps maintain pluripotency and self renewal via a STAT3 independent pathway. The introduction of Nanog, along with Sox2, Oct4, Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human Nanog-TAT is a 36.1 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13- residue C-terminal TAT peptide. |
Nanog-TAT Recombinant Protein |
40-526-002mg |
ProSci |
0.02 mg |
EUR 437.1 |
Description: Nanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of Nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of Nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, which helps maintain pluripotency and self renewal via a STAT3 independent pathway. The introduction of Nanog, along with Sox2, Oct4, Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human Nanog-TAT is a 36.1 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13- residue C-terminal TAT peptide. |
Lin28-TAT Recombinant Protein |
40-562-0005mg |
ProSci |
0.005 mg |
EUR 311.1 |
Description: Lin28 is a RNA-binding protein that belongs to a diverse family of structurally-related transcription factors. Lin28 is found abundantly in embryonic stem cells (ESCs), and to a lesser extent in placenta and testis. Lin28 has been shown to block let-7 microRNA processing and maturation, a necessary step in the differentiation of stem cells and certain cancer cell lines. Together with Sox2, Oct4, and Nanog, Lin28 can induce the reprogramming of primary human fibroblasts to a pluripotent state. Lin28 and other regulatory proteins can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing proteins into primary as well as transformed cells. Recombinant human Lin28-TAT is a 24.4 kDa protein containing 222 amino acid residues, including 13- residue C-terminal TAT peptide. |
Lin28-TAT Recombinant Protein |
40-562-0025mg |
ProSci |
0.025 mg |
EUR 437.1 |
Description: Lin28 is a RNA-binding protein that belongs to a diverse family of structurally-related transcription factors. Lin28 is found abundantly in embryonic stem cells (ESCs), and to a lesser extent in placenta and testis. Lin28 has been shown to block let-7 microRNA processing and maturation, a necessary step in the differentiation of stem cells and certain cancer cell lines. Together with Sox2, Oct4, and Nanog, Lin28 can induce the reprogramming of primary human fibroblasts to a pluripotent state. Lin28 and other regulatory proteins can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing proteins into primary as well as transformed cells. Recombinant human Lin28-TAT is a 24.4 kDa protein containing 222 amino acid residues, including 13- residue C-terminal TAT peptide. |
HIV1 tat antibody (FITC) |
60-00602-3F |
Fitzgerald |
50 ug |
EUR 648 |
Description: Rabbit polyclonal anti-tat HIV1 antibody (FITC) |
HIV1 tat antibody (biotin) |
60-00602-5 |
Fitzgerald |
50 ug |
EUR 548.4 |
Description: Rabbit polyclonal anti-tat HIV1 antibody (Biotin) |
HIV1 tat antibody (HRP) |
61-007002 |
Fitzgerald |
1 mg |
EUR 457.2 |
Description: Mouse monoclonal HIV1 tat antibody (HRP) |
HIV1 tat antibody (FITC) |
61-007003 |
Fitzgerald |
50 ug |
EUR 213.6 |
Description: Mouse monoclonal HIV1 tat antibody (FITC) |
HIV1 tat antibody (biotin) |
61-007005 |
Fitzgerald |
50 ug |
EUR 418.8 |
Description: Mouse monoclonal HIV1 tat antibody (biotin) |
TIGAR-TAT Recombinant Protein |
40-575-0005mg |
ProSci |
0.005 mg |
EUR 311.1 |
Description: TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells. Recombinant human TIGAR-TAT expressed in E. coli is a 36 kDa protein containing 284 amino-acid residues, including the 271 residues of full-length TIGAR fused to a 13-residue C-terminal peptide containing the TAT transduction domain (GGYGRKKRRQRRR). |
TIGAR-TAT Recombinant Protein |
40-575-0025mg |
ProSci |
0.025 mg |
EUR 437.1 |
Description: TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells. Recombinant human TIGAR-TAT expressed in E. coli is a 36 kDa protein containing 284 amino-acid residues, including the 271 residues of full-length TIGAR fused to a 13-residue C-terminal peptide containing the TAT transduction domain (GGYGRKKRRQRRR). |
Tyrosine Aminotransferase (TAT) Antibody |
20-abx131664 |
Abbexa |
-
EUR 526.80
-
EUR 159.60
-
EUR 1479.60
-
EUR 710.40
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Tyrosine Aminotransferase (TAT) Antibody |
20-abx131665 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1446.00
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
TIGAR-TAT (C12ORF5) Protein |
20-abx261124 |
Abbexa |
-
EUR 4101.60
-
EUR 393.60
-
EUR 276.00
|
|
|
Anti-ATTY/TAT Antibody |
A00622 |
BosterBio |
100ug/vial |
EUR 352.8 |
Active Tyrosine Aminotransferase (TAT) |
4-APD862Mu01 |
Cloud-Clone |
-
EUR 797.38
-
EUR 337.20
-
EUR 2660.16
-
EUR 966.72
-
EUR 1813.44
-
EUR 609.60
-
EUR 6470.40
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Tyrosine Aminotransferase expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
TAT (His) Lentiviral particles |
LVP019 |
GenTarget |
5x107 IFU/ml x 200ul |
EUR 418.8 |
Description: pre-made lentiviral particles expressing HIV TAT gene with N-term His tag |
Goat TAT ELISA Kit |
EGTT0509 |
Abclonal |
96Tests |
EUR 625.2 |
Anserini TAT ELISA Kit |
EAT0509 |
Abclonal |
96Tests |
EUR 625.2 |
The electrical characteristics of droplets can be modulated by controlling the number and type of layer used. Primary emulsions have the best resistance to various environmental conditions, while secondary emulsions have the worst, suggest electrostatic deposits may only be used to get certain functions. Interestingly, PLL regardless of the surface of the secondary emulsion at high salt concentrations due to electrostatic screening, which increases their salt stability. This phenomenon may be useful for several food applications, eg, have cationic droplets during food storage, but are anionic in the human body.