Objective: To determine the effect of electroacupuncture (EA) on intestinal Toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-kB) in obese mice, so as to explore the mechanism of action of acupuncture to lose weight. Methods: A total of 50 male Wistar rats were randomly divided into control and model of the group. high-fat feed used to establish a mouse model of obesity, and after modeling, 24 rats were randomly divided into model group, TLR4 inhibitor group and EA group, with 8 rats in each group.
Mice in group EA by EA at “Guanyuan” (CV4), “Zhongwan” (CV12), “Zusanli” (ST36), and “Fenglong” (ST40), 10 minutes each time, three times a week, and they were in a group of inhibitors TLR4 were given intraperitoneal injection of TAK-242 three times a week; course of treatment was 8 weeks for both groups. weight and the body’s blood sugar was measured every two weeks. Co-immunoprecipitation was used to observe the interaction between TLR4 and NF-kB p65 in colon tissue; electrophoretic mobility shift test is used to measure the activity of NF-kB p65; Western blot was used to measure the expression of TLR4 protein, phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (p-IκBα), and NF-kB p65; Quantitative real-time PCR was used to measure mRNA expression of TLR4, NF-kB p65 and IκBα.
Results: Compared with the control group, the model group had significant increases in weight, blood glucose, and protein and mRNA expression of TLR4 and NF-kB p65 (P <0.01, P <0.05), as well as a significant increase in interaction between TLR4 and NF-kB p65 and NF-kB p65 activity (P <0.05, P <0.01). Compared with the model group, EA group had a significant reduction in body weight (P <0.05), both groups EA and TLR4 inhibitor group had a significant reduction in blood glucose, and protein and mRNA expression of TLR4, p-IκBα and NF -kB p65 (P <0.05, P <0.01), as well as a significant reduction in the activity of NF-kB p65 (P <0.01). Conclusion: EA can effectively regulate TLR4 intestine, inhibits the interaction between TLR4 and NF-kB p65, and reduce the activity of NF-kB p65, which may be a potential mechanism of EA in reducing weight and blood glucose in obese mice.
[Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]
Orobol, derivatives of genistein, inhibits heat-killed Propionibacterium acnes-induced inflammation in HaCaT keratinocyte
Acne is a chronic skin disease that usually occurs in teens and twenties, and symptoms vary according to the age, sex, diet, and lifestyle. Acne is characterized by hyperproliferation of keratinocytes in the epidermis, excess sebum, the growth of Propionibacterium acnes, and P. acnes induced skin inflammation. Interleukin (IL) -1α and IL-6 were dominant in inflammatory lesions of acne vulgaris. This cytokine induces an inflammatory reaction of the skin in the presence of a pathogen or pressure.
Rat pIκBα(Phospho-Inhibitory Subunit of NF-KBα) ELISA Kit
RE1990R-48wellsplate
Reed Biotech
48-wells plate
EUR 126
Rat pIκBα(Phospho-Inhibitory Subunit of NF-KBα) ELISA Kit
RE1990R-96wellsplate
Reed Biotech
96-wells plate
EUR 180
Rat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2602067-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3290
Rat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2602067-48StripWells
MyBiosource
48-Strip-Wells
EUR 295
Rat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2602067-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1800
Rat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2602067-96StripWells
MyBiosource
96-Strip-Wells
EUR 450
Mouse phospho inhibitory subunit of NF kappaB alpha (pIKBα) ELISA Kit
NSL1139m
Sunlong
96 Test
EUR 528
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MBS2600700-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3545
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MBS2600700-48StripWells
MyBiosource
48-Strip-Wells
EUR 310
Goat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2600700-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1940
Goat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2600700-96StripWells
MyBiosource
96-Strip-Wells
EUR 475
Human Phospho inhibitory subunit of NF kappaB alpha(pIKBalpha) ELISA Kit
NSL0362Hu
Sunlong
96 wells
EUR 468
Mouse phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2600002-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3290
Mouse phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2600002-48StripWells
MyBiosource
48-Strip-Wells
EUR 295
Mouse phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2600002-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1800
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MBS2600002-96StripWells
MyBiosource
96-Strip-Wells
EUR 450
Human phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2602382-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3040
Human phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2602382-48StripWells
MyBiosource
48-Strip-Wells
EUR 285
Human phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2602382-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1670
Human phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2602382-96StripWells
MyBiosource
96-Strip-Wells
EUR 405
Human phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
EIA06756h
Enlibio
each
EUR 430
Bovine Phospho inhibitory subunit of NF kappaB alpha(pIKBalpha) ELISA Kit
NSL1168Bo
Sunlong
96 T
EUR 528
Canine Phospho inhibitory subunit of NF kappaB alpha(pIKBalpha) ELISA Kit
NSL1195Ca
Sunlong
1290 wells
EUR 528
Bovine phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2610154-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3545
Bovine phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2610154-48StripWells
MyBiosource
48-Strip-Wells
EUR 310
Bovine phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2610154-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1940
Bovine phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2610154-96StripWells
MyBiosource
96-Strip-Wells
EUR 475
Chicken phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2601068-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3545
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MBS2601068-48StripWells
MyBiosource
48-Strip-Wells
EUR 310
Chicken phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2601068-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1940
Chicken phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2601068-96StripWells
MyBiosource
96-Strip-Wells
EUR 475
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MBS2607938-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3545
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MBS2607938-48StripWells
MyBiosource
48-Strip-Wells
EUR 310
Canine phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2607938-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1940
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MBS2607938-96StripWells
MyBiosource
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EUR 475
Rabbit phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2602276-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3545
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MBS2602276-48StripWells
MyBiosource
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EUR 310
Rabbit phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2602276-5x96StripWells
MyBiosource
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EUR 1940
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MBS2602276-96StripWells
MyBiosource
96-Strip-Wells
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Porcine phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit
MBS2600056-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3545
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MBS2600056-48StripWells
MyBiosource
48-Strip-Wells
EUR 310
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MBS2600056-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1940
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MBS2600056-96StripWells
MyBiosource
96-Strip-Wells
EUR 475
Rat Phosphorylation Inhibitory Subunit Of NF-kappa B alpha ELISA Kit
MBS3809415-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 6725
Rat Phosphorylation Inhibitory Subunit Of NF-kappa B alpha ELISA Kit
MBS3809415-48StripWells
MyBiosource
48-Strip-Wells
EUR 550
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MBS3809415-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 3420
Rat Phosphorylation Inhibitory Subunit Of NF-kappa B alpha ELISA Kit
MBS3809415-96StripWells
MyBiosource
96-Strip-Wells
EUR 765
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MBS2601307-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3545
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MBS2601307-48StripWells
MyBiosource
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EUR 310
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MBS2601307-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1940
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MBS2601307-96StripWells
MyBiosource
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EUR 475
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MBS8426803-INQUIRE
MyBiosource
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MBS761825-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3900
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MBS761825-48StripWells
MyBiosource
48-Strip-Wells
EUR 340
Rat Inhibitory Subunit of NF-kappaBalpha ELISA Kit
MBS761825-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 2045
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MBS761825-96StripWells
MyBiosource
96-Strip-Wells
EUR 455
Rat Inhibitory Subunit Of NF Kappa B ELISA Kit
MBS3809622-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 6725
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MBS3809622-48StripWells
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48-Strip-Wells
EUR 550
Rat Inhibitory Subunit Of NF Kappa B ELISA Kit
MBS3809622-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 3420
Rat Inhibitory Subunit Of NF Kappa B ELISA Kit
MBS3809622-96StripWells
MyBiosource
96-Strip-Wells
EUR 765
Rat inhibitory subunit of NF-κBα(IKBα) Elisa Kit
EK720739
AFG Bioscience LLC
96 Wells
EUR 0.6
Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit
ER1084
FN Test
96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml
Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit
RE3437R-48wells
Reed Biotech
48 wells
EUR 125.55
Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit
RE3437R-96wells
Reed Biotech
96 wells
EUR 179.55
Rat inhibitory subunit of NF-κBα,IKBα ELISA Kit
SL0369Ra
Sunlong
-
EUR 498
Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit
RE3437R-48wellsplate
Reed Biotech
48-wells plate
EUR 126
Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit
RE3437R-96wellsplate
Reed Biotech
96-wells plate
EUR 180
Rat IKBα -Inhibitory Subunit of NF-κBα-CLIA Kit
E-CL-R0363-24Tests
Elabscience Biotech
24 Tests
EUR 180
Description: Sandwich
Rat IKBα -Inhibitory Subunit of NF-κBα-CLIA Kit
E-CL-R0363-48Tests
Elabscience Biotech
48 Tests
EUR 546
Description: Sandwich
Rat IKBα -Inhibitory Subunit of NF-κBα-CLIA Kit
E-CL-R0363-96Tests
Elabscience Biotech
96 Tests
EUR 682
Description: Sandwich
Rat IKBα -Inhibitory Subunit of NF-κBα-CLIA Kit
E-CL-R0363-96Tests10
Elabscience Biotech
96 Tests *10
EUR 6820
Description: Sandwich
Rat IKBα -Inhibitory Subunit of NF-κBα-CLIA Kit
E-CL-R0363-96Tests5
Elabscience Biotech
96 Tests *5
EUR 3410
Description: Sandwich
CLIA kit for Rat IKB? (Inhibitory Subunit of NF-?B?)
E-CL-R0363
Elabscience Biotech
1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Rat IKB? (Inhibitory Subunit of NF-?B?) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Rat IKB? (Inhibitory Subunit of NF-?B?)
E-EL-R0525
Elabscience Biotech
1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Rat IKB? (Inhibitory Subunit of NF-?B?) in samples from Serum, Plasma, Cell supernatant
Rat Inhibitory Subunit Of NF Kappa B beta ELISA Kit
MBS3809826-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 6725
Rat Inhibitory Subunit Of NF Kappa B beta ELISA Kit
MBS3809826-48StripWells
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48-Strip-Wells
EUR 550
Rat Inhibitory Subunit Of NF Kappa B beta ELISA Kit
MBS3809826-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 3420
Rat Inhibitory Subunit Of NF Kappa B beta ELISA Kit
MBS3809826-96StripWells
MyBiosource
96-Strip-Wells
EUR 765
Rat Inhibitory Subunit of NF-kB alpha (IKBa) CLIA Kit
abx197167-96tests
Abbexa
96 tests
EUR 990
Rat Inhibitory Subunit of NF Kappa B Alpha ELISA Kit
MBS091421-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 6725
Rat Inhibitory Subunit of NF Kappa B Alpha ELISA Kit
MBS091421-48StripWells
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48-Strip-Wells
EUR 550
Rat Inhibitory Subunit of NF Kappa B Alpha ELISA Kit
MBS091421-5x96StripWells
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5x96-Strip-Wells
EUR 3420
Rat Inhibitory Subunit of NF Kappa B Alpha ELISA Kit
MBS091421-96StripWells
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96-Strip-Wells
EUR 765
Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha) CLIA Kit
MBS2532659-5x96Tests
MyBiosource
5x96Tests
EUR 2565
Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha) CLIA Kit
MBS2532659-96Tests
MyBiosource
96Tests
EUR 560
Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit
MBS2512400-10x96Tests
MyBiosource
10x96Tests
EUR 4060
Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit
MBS2512400-24Tests
MyBiosource
24Tests
EUR 265
Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit
MBS2512400-48Tests
MyBiosource
48Tests
EUR 450
Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit
MBS2512400-5x96Tests
MyBiosource
5x96Tests
EUR 2265
Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit
MBS2512400-96Tests
MyBiosource
96Tests
EUR 535
Rat Inhibitory Subunit Of NF-kappaBalpha (IKBalpha) ELISA Kit
MBS3807965-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 6725
Rat Inhibitory Subunit Of NF-kappaBalpha (IKBalpha) ELISA Kit
MBS3807965-48StripWells
MyBiosource
48-Strip-Wells
EUR 550
Rat Inhibitory Subunit Of NF-kappaBalpha (IKBalpha) ELISA Kit
MBS3807965-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 3420
Rat Inhibitory Subunit Of NF-kappaBalpha (IKBalpha) ELISA Kit
MBS3807965-96StripWells
MyBiosource
96-Strip-Wells
EUR 765
Rat Inhibitory Subunit of NF-KBA Chemiluminescent Immunoassay Kit
IRTIKBAKTC
Innovative research
each
EUR 545
Description: Rat Inhibitory Subunit of NF-KBA Chemiluminescent Immunoassay Kit
Rat Inhibitory Subunit Of NF Kappa B Beta(IkBb)ELISA kit
QY-E10272
Qayee Biotechnology
96T
EUR 433.2
Rat Inhibitory Subunit Of NF Kappa B Beta (IkBb) ELISA Kit
MBS9311863-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 6725
Rat Inhibitory Subunit Of NF Kappa B Beta (IkBb) ELISA Kit
MBS9311863-48StripWells
MyBiosource
48-Strip-Wells
EUR 550
Rat Inhibitory Subunit Of NF Kappa B Beta (IkBb) ELISA Kit
MBS9311863-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 3420
Rat Inhibitory Subunit Of NF Kappa B Beta (IkBb) ELISA Kit
MBS9311863-96StripWells
MyBiosource
96-Strip-Wells
EUR 765
Rat Inhibitory Subunit Of NF Kappa B Alpha(IkBa)ELISA kit
QY-E10273
Qayee Biotechnology
96T
EUR 433.2
Human inhibitory subunit of NF-?B?,IKB? ELISA Kit
201-12-0683
Shanghai Sunred Biological Technology
96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Human inhibitory subunit of NF-κBα,IKBα ELISA Kit
YLA2357HU-48T
Shanghai YL Biotech
48T
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Human inhibitory subunit of NF-κBα,IKBα ELISA Kit
YLA2357HU-96T
Shanghai YL Biotech
96T
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Mouse inhibitory subunit of NF-κBα,IKBα ELISA Kit
YLA0368MO-48T
Shanghai YL Biotech
48T
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Mouse inhibitory subunit of NF-κBα,IKBα ELISA Kit
YLA0368MO-96T
Shanghai YL Biotech
96T
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Human inhibitory subunit of NF-κBα(IKBα)ELISA Kit
GA-E0699HM-48Tests
GenAsia Biotech
48 Tests
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Human inhibitory subunit of NF-κBα(IKBα)ELISA Kit
GA-E0699HM-96Tests
GenAsia Biotech
96 Tests
EUR 388
Mouse inhibitory subunit of NF-κBα(IKBα)ELISA Kit
GA-E0191MS-48Tests
GenAsia Biotech
48 Tests
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Mouse inhibitory subunit of NF-κBα(IKBα)ELISA Kit
GA-E0191MS-96Tests
GenAsia Biotech
96 Tests
EUR 460
Mouse inhibitory subunit of NF-κBα(IKBα)ELISA Kit
GA-E0191MS-48T
GenAsia Biotech
48T
EUR 403.2
Mouse inhibitory subunit of NF-κBα(IKBα)ELISA Kit
GA-E0191MS-96T
GenAsia Biotech
96T
EUR 640.8
Human inhibitory subunit of NF-κBα(IKBα)ELISA Kit
GA-E0699HM-48T
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48T
EUR 346.8
Human inhibitory subunit of NF-κBα(IKBα)ELISA Kit
GA-E0699HM-96T
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EUR 559.2
Cat Inhibitory Subunit of NF Kappa B Alpha ELISA Kit
MBS088616-INQUIRE
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Inhibitory Subunit Of NF Kappa B Zeta (IkBz) ELISA Kit
MBS2709495-10x96StripWells
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10x96-Strip-Wells
EUR 3215
Inhibitory Subunit Of NF Kappa B Zeta (IkBz) ELISA Kit
MBS2709495-24StripWells
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24-Strip-Wells
EUR 255
Inhibitory Subunit Of NF Kappa B Zeta (IkBz) ELISA Kit
MBS2709495-48StripWells
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48-Strip-Wells
EUR 340
Inhibitory Subunit Of NF Kappa B Zeta (IkBz) ELISA Kit
MBS2709495-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1675
Inhibitory Subunit Of NF Kappa B Zeta (IkBz) ELISA Kit
MBS2709495-96StripWells
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EUR 430
Cavy Inhibitory Subunit of NF Kappa B Alpha ELISA Kit
MBS105247-INQUIRE
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Goat Inhibitory Subunit of NF Kappa B Alpha ELISA Kit
MBS062479-INQUIRE
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In addition, IL-1α accelerate the production of keratin 16, which is normally expressed in the skin is injured or distorted, leading to abnormalities in the architecture and hyper-keratinization. 3 ‘, 4’, 5,7-Tetrahydroxyisoflavone, also termed orobol, is a metabolite of genistein. Orobol inhibit P. acnes induced increase in IL-6 and IL-1α levels in human keratinocytes (HaCaTs) compared with salicylic acid. Additionally, orobol decrease in IL-1α and IL-6 mRNA levels and inhibit phosphorylation of kinas e inhibitor of kappa-B, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, and mitogen-activated protein kinase induced by P. acnes , Finally, the expression of Ki67 decreased by orobol. Thus, improved orobol inflammation and hyper-keratinization caused by P. acnes dead heat and thus has the potential to be used in foods and cosmetics.