Objective: To determine the effect of electroacupuncture (EA) on intestinal Toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-kB) in obese mice, so as to explore the mechanism of action of acupuncture to lose weight. Methods: A total of 50 male Wistar rats were randomly divided into control and model of the group. high-fat feed used to establish a mouse model of obesity, and after modeling, 24 rats were randomly divided into model group, TLR4 inhibitor group and EA group, with 8 rats in each group.
Mice in group EA by EA at “Guanyuan” (CV4), “Zhongwan” (CV12), “Zusanli” (ST36), and “Fenglong” (ST40), 10 minutes each time, three times a week, and they were in a group of inhibitors TLR4 were given intraperitoneal injection of TAK-242 three times a week; course of treatment was 8 weeks for both groups. weight and the body’s blood sugar was measured every two weeks. Co-immunoprecipitation was used to observe the interaction between TLR4 and NF-kB p65 in colon tissue; electrophoretic mobility shift test is used to measure the activity of NF-kB p65; Western blot was used to measure the expression of TLR4 protein, phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (p-IκBα), and NF-kB p65; Quantitative real-time PCR was used to measure mRNA expression of TLR4, NF-kB p65 and IκBα.
Results: Compared with the control group, the model group had significant increases in weight, blood glucose, and protein and mRNA expression of TLR4 and NF-kB p65 (P <0.01, P <0.05), as well as a significant increase in interaction between TLR4 and NF-kB p65 and NF-kB p65 activity (P <0.05, P <0.01). Compared with the model group, EA group had a significant reduction in body weight (P <0.05), both groups EA and TLR4 inhibitor group had a significant reduction in blood glucose, and protein and mRNA expression of TLR4, p-IκBα and NF -kB p65 (P <0.05, P <0.01), as well as a significant reduction in the activity of NF-kB p65 (P <0.01). Conclusion: EA can effectively regulate TLR4 intestine, inhibits the interaction between TLR4 and NF-kB p65, and reduce the activity of NF-kB p65, which may be a potential mechanism of EA in reducing weight and blood glucose in obese mice.
[Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]
Orobol, derivatives of genistein, inhibits heat-killed Propionibacterium acnes-induced inflammation in HaCaT keratinocyte
Acne is a chronic skin disease that usually occurs in teens and twenties, and symptoms vary according to the age, sex, diet, and lifestyle. Acne is characterized by hyperproliferation of keratinocytes in the epidermis, excess sebum, the growth of Propionibacterium acnes, and P. acnes induced skin inflammation. Interleukin (IL) -1α and IL-6 were dominant in inflammatory lesions of acne vulgaris. This cytokine induces an inflammatory reaction of the skin in the presence of a pathogen or pressure.
Rat phospho-inhibitory subunit of NF-κBα(pIKBα)ELISA Kit
Description: A sandwich ELISA kit for quantitative measurement of Rat pI?B? (Phospho-Inhibitory Subunit of NF-KB?) in samples from Serum, Plasma, Cell supernatant
Description: A sandwich CLIA kit for quantitative measurement of Rat IKB? (Inhibitory Subunit of NF-?B?) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Rat IKB? (Inhibitory Subunit of NF-?B?)
Description: A sandwich ELISA kit for quantitative measurement of Rat IKB? (Inhibitory Subunit of NF-?B?) in samples from Serum, Plasma, Cell supernatant
Monkey Phospho-Inhibitory Subunit Of NF Kappa B Alpha (NFKB1) ELISA Kit
Description: A sandwich ELISA kit for quantitative measurement of Human pI?B? (Phospho-Inhibitory Subunit of NF Kappa B Alpha) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Mouse pI?B? (Phospho-Inhibitory Subunit of NF Kappa B Alpha)
Description: A sandwich ELISA kit for quantitative measurement of Mouse pI?B? (Phospho-Inhibitory Subunit of NF Kappa B Alpha) in samples from Serum, Plasma, Cell supernatant
Human Inhibitory Subunit of NF Kappa B Alpha (IκBa) CLIA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates or other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates or other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Inhibitory Subunit Of NF Kappa B Alpha ELISA Kit (IkBa)
Description: A sandwich ELISA kit for detection of Inhibitory Subunit Of NF Kappa B Alpha from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Mouse IkBa (Inhibitory Subunit Of NF Kappa B Alpha)
Description: A sandwich ELISA kit for detection of Inhibitory Subunit Of NF Kappa B Alpha from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Inhibitory Subunit Of NF Kappa B Alpha (IkBa) Polyclonal Antibody (Human)
Description: A sandwich CLIA kit for quantitative measurement of Human I?B? (Inhibitory Subunit of NF Kappa B Alpha) in samples from Serum, Plasma, Cell supernatant
High Sensitive for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)ELISA kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
High Sensitive for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)ELISA kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
High Sensitive for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)ELISA kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
High Sensitive for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)ELISA kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
High Sensitive ELISA Kit for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of High Sensitive Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
ELISA kit for Human I?B? (Inhibitory Subunit of NF Kappa B Alpha)
Description: A sandwich ELISA kit for quantitative measurement of Human I?B? (Inhibitory Subunit of NF Kappa B Alpha) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human I?B? (Inhibitory Subunit of NF Kappa B Beta)
Description: A sandwich ELISA kit for quantitative measurement of Human I?B? (Inhibitory Subunit of NF Kappa B Beta) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Mouse I?B? (Inhibitory Subunit of NF Kappa B Alpha)
Description: A sandwich ELISA kit for quantitative measurement of Mouse I?B? (Inhibitory Subunit of NF Kappa B Alpha) in samples from Serum, Plasma, Cell supernatant
Inhibitory Subunit Of NF Kappa B Alpha (IkBa) Polyclonal Antibody (Human), APC
Description: A Rabbit polyclonal antibody against Human Inhibitory Subunit Of NF Kappa B Beta (IkBb). This antibody is labeled with Biotin.
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In addition, IL-1α accelerate the production of keratin 16, which is normally expressed in the skin is injured or distorted, leading to abnormalities in the architecture and hyper-keratinization. 3 ‘, 4’, 5,7-Tetrahydroxyisoflavone, also termed orobol, is a metabolite of genistein. Orobol inhibit P. acnes induced increase in IL-6 and IL-1α levels in human keratinocytes (HaCaTs) compared with salicylic acid. Additionally, orobol decrease in IL-1α and IL-6 mRNA levels and inhibit phosphorylation of kinase inhibitor of kappa-B, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, and mitogen-activated protein kinase induced by P. acnes , Finally, the expression of Ki67 decreased by orobol. Thus, improved orobol inflammation and hyper-keratinization caused by P. acnes dead heat and thus has the potential to be used in foods and cosmetics.
![[Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]](https://ikb-a.com/wp-content/uploads/2020/08/photo_2020-08-08_23-07-35-3.jpg)
![[Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]](https://ikb-a.com/wp-content/uploads/2020/08/photo_2020-08-08_23-07-35-3-484x1024.jpg)
