Objective: To determine the effect of electroacupuncture (EA) on intestinal Toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-kB) in obese mice, so as to explore the mechanism of action of acupuncture to lose weight. Methods: A total of 50 male Wistar rats were randomly divided into control and model of the group. high-fat feed used to establish a mouse model of obesity, and after modeling, 24 rats were randomly divided into model group, TLR4 inhibitor group and EA group, with 8 rats in each group.
Mice in group EA by EA at “Guanyuan” (CV4), “Zhongwan” (CV12), “Zusanli” (ST36), and “Fenglong” (ST40), 10 minutes each time, three times a week, and they were in a group of inhibitors TLR4 were given intraperitoneal injection of TAK-242 three times a week; course of treatment was 8 weeks for both groups. weight and the body’s blood sugar was measured every two weeks. Co-immunoprecipitation was used to observe the interaction between TLR4 and NF-kB p65 in colon tissue; electrophoretic mobility shift test is used to measure the activity of NF-kB p65; Western blot was used to measure the expression of TLR4 protein, phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (p-IκBα), and NF-kB p65; Quantitative real-time PCR was used to measure mRNA expression of TLR4, NF-kB p65 and IκBα.
Results: Compared with the control group, the model group had significant increases in weight, blood glucose, and protein and mRNA expression of TLR4 and NF-kB p65 (P <0.01, P <0.05), as well as a significant increase in interaction between TLR4 and NF-kB p65 and NF-kB p65 activity (P <0.05, P <0.01). Compared with the model group, EA group had a significant reduction in body weight (P <0.05), both groups EA and TLR4 inhibitor group had a significant reduction in blood glucose, and protein and mRNA expression of TLR4, p-IκBα and NF -kB p65 (P <0.05, P <0.01), as well as a significant reduction in the activity of NF-kB p65 (P <0.01). Conclusion: EA can effectively regulate TLR4 intestine, inhibits the interaction between TLR4 and NF-kB p65, and reduce the activity of NF-kB p65, which may be a potential mechanism of EA in reducing weight and blood glucose in obese mice.
Orobol, derivatives of genistein, inhibits heat-killed Propionibacterium acnes-induced inflammation in HaCaT keratinocyte
Acne is a chronic skin disease that usually occurs in teens and twenties, and symptoms vary according to the age, sex, diet, and lifestyle. Acne is characterized by hyperproliferation of keratinocytes in the epidermis, excess sebum, the growth of Propionibacterium acnes, and P. acnes induced skin inflammation. Interleukin (IL) -1α and IL-6 were dominant in inflammatory lesions of acne vulgaris. This cytokine induces an inflammatory reaction of the skin in the presence of a pathogen or pressure.
Human Phospho-Inhibitory Subunit (pIκBα) ELISA Kit
Description: A sandwich CLIA kit for quantitative measurement of Rat IKB? (Inhibitory Subunit of NF-?B?) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Rat IKB? (Inhibitory Subunit of NF-?B?)
Description: A sandwich ELISA kit for quantitative measurement of Rat IKB? (Inhibitory Subunit of NF-?B?) in samples from Serum, Plasma, Cell supernatant
Rat Inhibitory Subunit of NF-kB alpha (IKBa) CLIA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates or other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates or other biological fluids.
Mouse IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IkBa in the samples is then determined by comparing the OD of the samples to the standard curve.
Mouse IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IkBa in the samples is then determined by comparing the OD of the samples to the standard curve.
Human IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IkBa in the samples is then determined by comparing the OD of the samples to the standard curve.
Human IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IkBa in the samples is then determined by comparing the OD of the samples to the standard curve.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
In addition, IL-1α accelerate the production of keratin 16, which is normally expressed in the skin is injured or distorted, leading to abnormalities in the architecture and hyper-keratinization. 3 ‘, 4’, 5,7-Tetrahydroxyisoflavone, also termed orobol, is a metabolite of genistein. Orobol inhibit P. acnes induced increase in IL-6 and IL-1α levels in human keratinocytes (HaCaTs) compared with salicylic acid. Additionally, orobol decrease in IL-1α and IL-6 mRNA levels and inhibit phosphorylation of kinase inhibitor of kappa-B, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, and mitogen-activated protein kinase induced by P. acnes , Finally, the expression of Ki67 decreased by orobol. Thus, improved orobol inflammation and hyper-keratinization caused by P. acnes dead heat and thus has the potential to be used in foods and cosmetics.