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[Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]

[Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]
Posted by Anna

Objective: To determine the effect of electroacupuncture (EA) on intestinal Toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-kB) in obese mice, so as to explore the mechanism of action of acupuncture to lose weight.
Methods: A total of 50 male Wistar rats were randomly divided into control and model of the group. high-fat feed used to establish a mouse model of obesity, and after modeling, 24 rats were randomly divided into model group, TLR4 inhibitor group and EA group, with 8 rats in each group.

Mice in group EA by EA at “Guanyuan” (CV4), “Zhongwan” (CV12), “Zusanli” (ST36), and “Fenglong” (ST40), 10 minutes each time, three times a week, and they were in a group of inhibitors TLR4 were given intraperitoneal injection of TAK-242 three times a week; course of treatment was 8 weeks for both groups. weight and the body’s blood sugar was measured every two weeks. Co-immunoprecipitation was used to observe the interaction between TLR4 and NF-kB p65 in colon tissue; electrophoretic mobility shift test is used to measure the activity of NF-kB p65; Western blot was used to measure the expression of TLR4 protein, phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (p-IκBα), and NF-kB p65; Quantitative real-time PCR was used to measure mRNA expression of TLR4, NF-kB p65 and IκBα.


Results: Compared with the control group, the model group had significant increases in weight, blood glucose, and protein and mRNA expression of TLR4 and NF-kB p65 (P <0.01, P <0.05), as well as a significant increase in interaction between TLR4 and NF-kB p65 and NF-kB p65 activity (P <0.05, P <0.01). Compared with the model group, EA group had a significant reduction in body weight (P <0.05), both groups EA and TLR4 inhibitor group had a significant reduction in blood glucose, and protein and mRNA expression of TLR4, p-IκBα and NF -kB p65 (P <0.05, P <0.01), as well as a significant reduction in the activity of NF-kB p65 (P <0.01).
Conclusion: EA can effectively regulate TLR4 intestine, inhibits the interaction between TLR4 and NF-kB p65, and reduce the activity of NF-kB p65, which may be a potential mechanism of EA in reducing weight and blood glucose in obese mice.

[Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]
[Effect of electroacupuncture on intestinal Toll-like receptor 4 and nuclear factor-kappa B in obese rats]

Orobol, derivatives of genistein, inhibits heat-killed Propionibacterium acnes-induced inflammation in HaCaT keratinocyte

Acne is a chronic skin disease that usually occurs in teens and twenties, and symptoms vary according to the age, sex, diet, and lifestyle. Acne is characterized by hyperproliferation of keratinocytes in the epidermis, excess sebum, the growth of Propionibacterium acnes, and P. acnes induced skin inflammation. Interleukin (IL) -1α and IL-6 were dominant in inflammatory lesions of acne vulgaris. This cytokine induces an inflammatory reaction of the skin in the presence of a pathogen or pressure.

Rat pIκBα(Phospho-Inhibitory Subunit of NF-KBα) ELISA Kit

RE1990R-48wellsplate 48-wells plate
EUR 126

Rat pIκBα(Phospho-Inhibitory Subunit of NF-KBα) ELISA Kit

RE1990R-96wellsplate 96-wells plate
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Rat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

MBS2602067-10x96StripWells 10x96-Strip-Wells
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Rat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Rat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Rat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Human Phospho-Inhibitory Subunit (pIκBα) ELISA Kit

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Mouse phospho inhibitory subunit of NF kappaB alpha (pIKBα) ELISA Kit

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Goat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Goat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Goat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Goat phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Human Phospho inhibitory subunit of NF kappaB alpha(pIKBalpha) ELISA Kit

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Mouse phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Mouse phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Mouse phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Human phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Human phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

MBS2602382-48StripWells 48-Strip-Wells
EUR 285

Human phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Human phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Human phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

EIA06756h each
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Canine Phospho inhibitory subunit of NF kappaB alpha(pIKBalpha) ELISA Kit

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Bovine phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Bovine phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Bovine phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Chicken phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Chicken phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Chicken phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Canine phospho inhibitory subunit of NF kappaB alpha (pIKBalpha) ELISA Kit

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Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit

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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml

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Description: Sandwich

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Description: Sandwich

Rat IKBα -Inhibitory Subunit of NF-κBα-CLIA Kit

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Rat IKBα -Inhibitory Subunit of NF-κBα-CLIA Kit

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Description: Sandwich

Rat IKBα -Inhibitory Subunit of NF-κBα-CLIA Kit

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CLIA kit for Rat IKB? (Inhibitory Subunit of NF-?B?)

E-CL-R0363 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Rat IKB? (Inhibitory Subunit of NF-?B?) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat IKB? (Inhibitory Subunit of NF-?B?)

E-EL-R0525 1 plate of 96 wells
EUR 640.8
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Rat Inhibitory Subunit Of NF Kappa B beta ELISA Kit

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Rat Inhibitory Subunit Of NF Kappa B beta ELISA Kit

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Rat Inhibitory Subunit Of NF Kappa B beta ELISA Kit

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Rat Inhibitory Subunit Of NF Kappa B beta ELISA Kit

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Rat Inhibitory Subunit of NF-kB alpha (IKBa) CLIA Kit

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Rat Inhibitory Subunit of NF Kappa B Alpha ELISA Kit

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Rat Inhibitory Subunit of NF Kappa B Alpha ELISA Kit

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Rat Inhibitory Subunit of NF Kappa B Alpha ELISA Kit

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Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha) CLIA Kit

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Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit

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Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit

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Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit

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Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit

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Rat Inhibitory Subunit Of NF-kappaBalpha (IKBalpha) ELISA Kit

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Rat Inhibitory Subunit Of NF-kappaBalpha (IKBalpha) ELISA Kit

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Rat Inhibitory Subunit Of NF-kappaBalpha (IKBalpha) ELISA Kit

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Description: Rat Inhibitory Subunit of NF-KBA Chemiluminescent Immunoassay Kit

Rat Inhibitory Subunit Of NF Kappa B Beta(IkBb)ELISA kit

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Rat Inhibitory Subunit Of NF Kappa B Beta (IkBb) ELISA Kit

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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

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In addition, IL-1α accelerate the production of keratin 16, which is normally expressed in the skin is injured or distorted, leading to abnormalities in the architecture and hyper-keratinization. 3 ‘, 4’, 5,7-Tetrahydroxyisoflavone, also termed orobol, is a metabolite of genistein. Orobol inhibit P. acnes induced increase in IL-6 and IL-1α levels in human keratinocytes (HaCaTs) compared with salicylic acid. Additionally, orobol decrease in IL-1α and IL-6 mRNA levels and inhibit phosphorylation of kinase inhibitor of kappa-B, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, and mitogen-activated protein kinase induced by P. acnes , Finally, the expression of Ki67 decreased by orobol. Thus, improved orobol inflammation and hyper-keratinization caused by P. acnes dead heat and thus has the potential to be used in foods and cosmetics.

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