In osteoarthritis (OA), excessive inhibition of pro-inflammatory cytokines expressed in OA joints and increase anabolism for cartilage regeneration is required. In a study of ex-vivo, we used a model of inflammatory chondrocytes OA microtissues humans, which consists of treatment with cytokines (interleukin 1β (IL-1β) / tumor necrosis factor α (TNF-α)) with or without supplementation six herbal compounds with effects chondroprotective identified. The compounds were assessed for their capacity to modulate catabolic and anabolic key factors by using several molecular analysis.
We selectively investigate the mechanism of action of the two most potent compounds of vanillic acid (VA) and Epimedin C (Epi C). After the identification of anti-inflammatory properties and anabolic of VA and Epi C, Ingenuity Pathway Analysis showed that in both treatment groups, osteoarthritic signaling pathway inhibition. In the group treated with the VA, nuclear factor kappa-light-chain-enhancer activated B cells (NF-kB) signaling is inhibited by the weakening of the nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκBα) phosphorylation.
Epi C showed a significant anabolic effect by increasing the expression of matrix proteins collagen and non-collagen. In conclusion, VA, through inhibition of phosphorylation at NF-kB signaling pathway and Epi C, with increased expression of extracellular matrix components, demonstrated anti-inflammatory properties and a significant anabolic and may potentially be used in combination to treat or prevent OA joints.
Explanation colitis stomach-protective efficacy of diosgenin in TNBS-induced experimental: inhibition of NF-kB / IKB-α and Bax / Caspase-1 signaling pathway
The purpose of this study is to explain the beneficial role of diosgenin unfold against experimental models of TNBS (2,4,6-trinitrobenzenesufonic acid) -induced ulcerative colitis (UC). Induced colitis in rats Sprague-Dawley with intrarectal administration of TNBS (in 50% ethanol). Then the animals were treated with diosgenin (50, 100, and 200 mg / kg) for 14 days. Various biochemical, behavioral, molecular and histological analysis performed.
Diosgenin decreased significantly (p <BS-induced increase in colonic Oxido-nitrosative damage, myeloperoxidase, hydroxyproline, mRNA expression of proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IFN-γ) and inflammatory markers (iNOS and COX -2) caused by TNBS. Western blot analysis relevated the TNBS-induced up-regulated the expression of NF-kB protein, IκBα, Bax, and Caspase-1 were markedly decreased (p <treatment. it is also evident repaired insult induced histological in the gut by TNBS. in conclusion, given diosgenin gut-protective properties that may be through inhibition of NF-kB / IKB-α and Bax / Caspase-1 signaling pathways for TNBS-induced experimental ulcerative colitis.
Human IκBα -Inhibitory Subunit of NF Kappa B Alpha- CLIA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IkBa in the samples is then determined by comparing the OD of the samples to the standard curve.
Mouse IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IkBa in the samples is then determined by comparing the OD of the samples to the standard curve.
Human IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IkBa in the samples is then determined by comparing the OD of the samples to the standard curve.
Human IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IkBa in the samples is then determined by comparing the OD of the samples to the standard curve.
Human Inhibitory Subunit Of NF Kappa B Alpha ELISA Kit (IkBa)
Description: A sandwich quantitative ELISA assay kit for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates or other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates or other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.