In osteoarthritis (OA), excessive inhibition of pro-inflammatory cytokines expressed in OA joints and increase anabolism for cartilage regeneration is required. In a study of ex-vivo, we used a model of inflammatory chondrocytes OA microtissues humans, which consists of treatment with cytokines (interleukin 1β (IL-1β) / tumor necrosis factor α (TNF-α)) with or without supplementation six herbal compounds with effects chondroprotective identified. The compounds were assessed for their capacity to modulate catabolic and anabolic key factors by using several molecular analysis.
We selectively investigate the mechanism of action of the two most potent compounds of vanillic acid (VA) and Epimedin C (Epi C). After the identification of anti-inflammatory properties and anabolic of VA and Epi C, Ingenuity Pathway Analysis showed that in both treatment groups, osteoarthritic signaling pathway inhibition. In the group treated with the VA, nuclear factor kappa-light-chain-enhancer activated B cells (NF-kB) signaling is inhibited by the weakening of the nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκBα) phosphorylation.
Epi C showed a significant anabolic effect by increasing the expression of matrix proteins collagen and non-collagen. In conclusion, VA, through inhibition of phosphorylation at NF-kB signaling pathway and Epi C, with increased expression of extracellular matrix components, demonstrated anti-inflammatory properties and a significant anabolic and may potentially be used in combination to treat or prevent OA joints.
Anti-Inflammatory and Chondroprotective Effects of Vanillic Acid and Epimedin C in Human Osteoarthritic Chondrocytes
Explanation colitis stomach-protective efficacy of diosgenin in TNBS-induced experimental: inhibition of NF-kB / IKB-α and Bax / Caspase-1 signaling pathway
The purpose of this study is to explain the beneficial role of diosgenin unfold against experimental models of TNBS (2,4,6-trinitrobenzenesufonic acid) -induced ulcerative colitis (UC). Induced colitis in rats Sprague-Dawley with intrarectal administration of TNBS (in 50% ethanol). Then the animals were treated with diosgenin (50, 100, and 200 mg / kg) for 14 days. Various biochemical, behavioral, molecular and histological analysis performed.
Diosgenin decreased significantly (p <BS-induced increase in colonic Oxido-nitrosative damage, myeloperoxidase, hydroxyproline, mRNA expression of proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IFN-γ) and inflammatory markers (iNOS and COX -2) caused by TNBS. Western blot analysis relevated the TNBS-induced up-regulated the expression of NF-kB protein, IκBα, Bax, and Caspase-1 were markedly decreased (p <treatment. it is also evident repaired insult induced histological in the gut by TNBS. in conclusion, given diosgenin gut-protective properties that may be through inhibition of NF-kB / IKB-α and Bax / Caspase-1 signaling pathways for TNBS-induced experimental ulcerative colitis.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates or other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates or other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) ELISA Kit
Description: A sandwich ELISA kit for quantitative measurement of Human I?B? (Inhibitory Subunit of NF Kappa B Alpha) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Mouse I?B? (Inhibitory Subunit of NF Kappa B Alpha)
Description: A sandwich ELISA kit for quantitative measurement of Mouse I?B? (Inhibitory Subunit of NF Kappa B Alpha) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Mouse IkBa (Inhibitory Subunit Of NF Kappa B Alpha)
Description: A sandwich ELISA kit for detection of Inhibitory Subunit Of NF Kappa B Alpha from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Human IkBa (Inhibitory Subunit Of NF Kappa B Alpha)
Description: A sandwich ELISA kit for detection of Inhibitory Subunit Of NF Kappa B Alpha from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Inhibitory Subunit of NF Kappa B Alpha (IkBa) Antibody
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
High Sensitive for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)ELISA kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
High Sensitive for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)ELISA kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
High Sensitive for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)ELISA kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in Tissue homogenates, cell lysates and other biological fluids.
High Sensitive ELISA Kit for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of High Sensitive Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Human Inhibitory Subunit Of NF Kappa B Alpha (IkBa) Protein
Description: A sandwich CLIA kit for quantitative measurement of Human I?B? (Inhibitory Subunit of NF Kappa B Alpha) in samples from Serum, Plasma, Cell supernatant
Inhibitory Subunit of NF Kappa B Alpha (IkBa) Antibody (Biotin)
Description: A sandwich ELISA kit for quantitative measurement of Human I?B? (Inhibitory Subunit of NF Kappa B Beta) in samples from Serum, Plasma, Cell supernatant
Inhibitory Subunit Of NF Kappa B Alpha (IkBa) Polyclonal Antibody (Human), APC-Cy7
Description: A competitive ELISA for quantitative measurement of Rabbit NF kappa B inhibitor Alpha (NFKBIA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit NF kappa B inhibitor Alpha (NFKBIA) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit NF kappa B inhibitor Alpha (NFKBIA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit NF kappa B inhibitor Alpha (NFKBIA) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit NF kappa B inhibitor Alpha (NFKBIA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Inhibitory Subunit Of NF Kappa B Epsilon (IkBe) Polyclonal Antibody (Human), APC